During Spring Training, there is no limit for how many players a Major League Baseball team can have but during the regular season all 30 teams will only have 25 players on their roster unless the 26th man rule is in event for a double header between two games. Starting on September 1st, teams can up players from their Minor League teams so that they can have a total of 40 players on their roster. During the Postseason only 25 players are allowed on a roster.
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According to baseball-reference.com, none. Odd, huh?
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There is one Major League Baseball team, the Miami Marlins.