There is no set date, but its almost indelible tradition is to play it on the last Saturday in September each year. In this year of 2010, it was a draw on that day, so it was rescheduled to be played again the very next week - the first Saturday in October.
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The first games played in the AFL (Australian Football League) began at about 2.10 pm on Saturday 31 March 1990. Four matches took place that afternoon at various grounds in Melbourne.
If you were meaning, "When was the first league football match, as in the VFL, or other state league?" the Victorian Football League played its first matches in 1896.
However, there are records of the very early beginnings of Australian football back in the 1850's, possibly earlier. Scotch College, Melbourne Grammar, Geelong Grammar and a St. Kilda school all indicate in the early beginnings of the game.
The first match of the AFL season for 2011 is Thursday March 24th. Between Richmond and Carlton at the MCG. 7:40pm start.
The season has already commenced.It began Friday, 22nd March - with the Essendon Bombers defeating the Adelaide Crows in Adelaide - that being the only game of Opening night.