Johnny Rhodes from Maryland (1992-96) with 344. He also has the ACC record for steals per game for his career (2.82), steals in a single season (110), and steals per game for a single season (3.7).
See this 2012 ACC press release for reference: http://www.theacc.com/sports/m-baskbl/spec-rel/020912aaa.html
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There have been many great defensive players in college Basketball history. However, the NCAA all-time steals leader is a guy named John Linehan who had 385 steals in 1997 with Providence.