Any loss to Iowa State is arguably the worst loss ever.
Wisconsin
FSU destroys Michigan at Michigan. 51-31
As of now, the worst football team in the league is subjective and can vary based on different factors such as win-loss record, performance, and statistics.
In Bryant Denny Stadium: 1951 Villanova 41 - Alabama 18
130-0 vs Michigan on October 22, 1904.
1996 they lost to Ohio State 72-0.
Missouri's worst loss in football occurred on October 31, 1975, when they were defeated by the University of Kansas with a score of 55-0. This game marked a significant low point for the program, as it was a humiliating defeat against their longstanding rival. The loss is often remembered as one of the most lopsided games in Missouri's football history.
Through the 2008 season, Penn State and Texas Tech have met once in football. That was September 9, 1995 and Penn State won, 24-23, in State College.
The loss of dignity and innocence for the whole program in 2011. Or the 79 Sugar Bowl.
2009 Big 12 championship before the referees decided they would rather have Texas in the National championship than obeying the rule book, which explicitly states that the end of game is not a reviewable play. or their loss to Texas Tech in 2008 due to a last minute play by Crabtree.
I don't know what is their worst ever, but in the 13th of September 1997, Texas had an embarrassing 66 - 3 lost to UCLA. that's the worst that I can remember. I believe that their worst loss came in 1999, when they lost to Nebraska 56-3. In 1998, they lost to Texas 58-7; in 1986 they lost to Miami (Fla) 61-11. Those, to my knowledge, are the only 50+ point losses by Texas Tech. Their biggest winning margin was in 1925; they beat Wayland Baptist 120-0. In modern times, they beat Nebraska 70-10 (2004), Northwestern State 75-7 (2007) and S/E Louisiana 62-0 (2006).