As far as all time winning percentages go, through 15 games of the 2009 season that would be the Houston Texans who have an all time regular season record of 48-79 for a winning percentage of .378.
The Tampa Bay Buccaneers have the second lowest all time regular season winning percentage at .393 (208-322-1) and the Atlanta Falcons are third lowest at .414 (275-390-6).
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The Detroit Lions had a record of 0-16 last season making them the worst team in the league.
To be honest, I think the worst are West Ham United and all the teams in the Artricity League. West Ham are also flukey and most off their wins are by fluke