The runner from 2nd is only out when the defensive team tags 2nd base, not when the infraction occurs. So the run will count unless the defensive team had tagged 2nd base before the run crossed the plate.
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Not immediatly. If you are running to first base and beat the ball there, but you miss the bag, I can tag you out if I have the ball. I CANNOT get you out by tagging first on that play. I have to wait for the next play.
No, this is an appeal call only. This is not the umpires call unless asked on appeal.
YES he can be called out on an appeal... this would be the same as if a home was hit and the runner had not tagged first base ...