The hockey stick spreads the known concentration of bacteria evenly over the agar plate.
In microbiology, a hockey stick is a tool used for streaking bacterial cultures on agar plates to isolate individual colonies. By dragging the stick across the plate, the bacteria are spread out in a way that allows for distinct colonies to grow. This helps microbiologists study and identify different strains of bacteria.
It is necessary to make the colonies well-isolated from each other so that each appears distinct, large and shows characteristic growth forms.Most bacteria, many other microfungi, and unicellular microalgae, may be most commonly obtained by plating methods such as streak plate method, pour plate method and spread plate method.
Previous answer: "because of infection" This person is obviously trying to be funny, the right word should be "Contamination", as for spread plate, the bacteria is more exposed to air as it is spread over the agar plate. Therefore, the result might not be accurate, as it might be contaminated. As for the pour plate method, the bacteria is in the agar itself, it is not exposed to air, thus, less risk of getting contaminated.
Rober Kock developed the culture plate method to identify pathogens.
The plating technique most likely performed when using the dilution technique is spread plating. In spread plating, a sample is spread over the surface of the agar plate using a sterile spreading tool to obtain individual colonies. This method helps to isolate and quantify bacteria present in the sample.
In the pour plate, the microorganisms will grow within the gel that has been set, and in the spread-plate technique, growth will be on top of the agar gel where it has been spread.
In microbiology, a hockey stick is a tool used for streaking bacterial cultures on agar plates to isolate individual colonies. By dragging the stick across the plate, the bacteria are spread out in a way that allows for distinct colonies to grow. This helps microbiologists study and identify different strains of bacteria.
Both Spread-plate and pour plate method don't give the same results. Because in the case of spread plate method the inoculmn used for inoculation can't be spread in a exact volume. A little inoculmn remains stick with the spreader after spreading. On the other hand, in pour plate method it doesn't happen. So mostly, through comparing the counts by both methods, less counts are obtained in spread plate method. I am Working as a Sr. Microbiologist in a Biotech company
It is necessary to make the colonies well-isolated from each other so that each appears distinct, large and shows characteristic growth forms.Most bacteria, many other microfungi, and unicellular microalgae, may be most commonly obtained by plating methods such as streak plate method, pour plate method and spread plate method.
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Previous answer: "because of infection" This person is obviously trying to be funny, the right word should be "Contamination", as for spread plate, the bacteria is more exposed to air as it is spread over the agar plate. Therefore, the result might not be accurate, as it might be contaminated. As for the pour plate method, the bacteria is in the agar itself, it is not exposed to air, thus, less risk of getting contaminated.
The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.
Rober Kock developed the culture plate method to identify pathogens.
it is the plate under the plate (kea nga UNDER plate!!!) hahaha
You may be referring to a " hockey stick. " This is a short glass rod bent in a hockey stick shape and it is used to spread cells on a cell plate after they have been delivered to the plate by microliter pipette.
Rober Kock developed the culture plate method to identify pathogens.
1 The Spread Plate: If a mixture of cells is spread out on an agar surface so that every cell grows into a completely separate colony, a macroscopically visible growth or cluster of microorganisms on a solid medium, each colony represents a pure culture. The spread plate is an easy, direct way of achieving this 2 The Pour Plate: Extensively used with bacteria and fungi, a pour plate also can yield isolated colonies. The original sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating result. 3 The streak plate: Pure colonies also can be obtained from streak plates. The microbial mixture is transferred to the edge of an agar plate with an inoculating loop or swab and then streaked out over the surface in several patterns