UNC was a number 1 seed in the 2009 NCAA Basketball tournament.
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The Louisville Cardinals are the overall number one seed in the 2009 NCAA tournament and will be playing in the Midwest Region
Kansas
Villanova was an eighth seed in 1985.
The earliest round for a 1 seed to go out in the NCAA basketball tourney is in the first round. It happened in 2018 when the University of Maryland - Baltimore County (16th seed in the South) beat the University of Virginia (1st seed in the South) by a score of 74 - 54.
Connecticut in 2004