Lady Jane Grey, Anne Boleyn & Catherine Howard spring to mind.....
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Two of Henry VIII's queens were beheaded - but not by him personally. His second queen, Anne Boleyn (mother of Queen Elizabeth I) was beheaded, and his fifth queen, Katherine Howard was beheaded.
2 Another answer Since King Offa in 774 and King William III in 1702 there have been about 53 Kings of England. After 1707 and the Union with Scotland, Kings and Queens ruled Great Britain. The link below had more information about the list of English Monarchs
The Tower of London.
King Charles I (1600-1649) was beheaded on January 30, 1649, following the English Civil War.
Oliver Cromwell, after Charles l was beheaded.