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Yes, the first Kentucky Derby included fillies among its participating horses. There were 13 colts and two fillies. The two chestnut fillies were Ascension and Gold Mine.

Ascension placed 10th. She was ridden by Billy Lakeland, the only white rider among a total of 15 jockeys.

Gold Mine finished last. She was ridden by Stradford.

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Q: Did the first Kentucky Derby have any fillies?
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