Yes. On December 16, 2012, the Dallas Cowboys defeated the Steelers by the score of 27-24 in overtime.
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The Dallas Cowboys won five Super Bowls, these were the teams they defeated:1/16/1972 - Miami Dolphins1/15/1978 - Denver Broncos1/31/1993 - Buffalo Bills1/30/1994 - Buffalo Bills1/28/1996 - Pittsburgh Steelers
SB IX: Steelers 16, Vikings 6 SB X: Steelers 21, Cowboys 17 SB XIII: Steelers 35, Cowboys 31 SB XIV: Steelers 31, Rams 19 SB XL: Steelers 21, Seahawks 10 SB XLIII: Steelers 27, Cardinals 23
It was the Jacksonville Jaguars in the 2007 season. They defeated the Steelers at Heinz Field on December 16, 2007 and also on January 5, 2008 in the AFC Wild Card game.
The Pittsburgh Steelers and the Green Bay Packers will be in Super Bowl 45. Steelers will defeat the New York Jets 22-17 in the AFC Championship game. Packers will defeat the Chicago Bears 21-14 in the NFC Championship game. Super Bowl 45 will be held in Arlington, Texas on Sunday, February 6, 2011.
No, the Cowboys would then need to defeat the winner of the New Orleans Saints-Arizona Cardinals game in the NFC Championship game on January 24 to advance to the Super Bowl.