Yes. On December 16, 2012, the Dallas Cowboys defeated the Steelers by the score of 27-24 in overtime.
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The Dallas Cowboys won five Super Bowls, these were the teams they defeated:1/16/1972 - Miami Dolphins1/15/1978 - Denver Broncos1/31/1993 - Buffalo Bills1/30/1994 - Buffalo Bills1/28/1996 - Pittsburgh Steelers
SB IX: Steelers 16, Vikings 6 SB X: Steelers 21, Cowboys 17 SB XIII: Steelers 35, Cowboys 31 SB XIV: Steelers 31, Rams 19 SB XL: Steelers 21, Seahawks 10 SB XLIII: Steelers 27, Cardinals 23
It was the Jacksonville Jaguars in the 2007 season. They defeated the Steelers at Heinz Field on December 16, 2007 and also on January 5, 2008 in the AFC Wild Card game.
The Pittsburgh Steelers and the Green Bay Packers will be in Super Bowl 45. Steelers will defeat the New York Jets 22-17 in the AFC Championship game. Packers will defeat the Chicago Bears 21-14 in the NFC Championship game. Super Bowl 45 will be held in Arlington, Texas on Sunday, February 6, 2011.
Through the 2012 season, the Steelers have played the Titans 75 times, from the Titans' days as the Houston Oilers. The Steelers won 34 regular-season games and three playoff games against the Houston Oilers in the period of 1970-96, but from 1997, when the Oilers moved to Tennessee, the Steelers have won just six times as opposed to ten regular-season wins and one playoff win over the Steelers by the Titans. Some notable games - January 1979 AFC Playoffs - Steelers defeat Oilers 34-5 December 2, 1984 - Oilers defeat Steelers 20-17 December 31, 1989 - AFC Playoffs - Steelers defeat Oilers 26-23 in OT September 29, 1996 - Steelers defeat Oilers 30-16 - two brawls erupt during the game and several players from both clubs were ejected. November 17, 2002 - Titans defeat Steelers 31-23 - Tommy Maddox suffered momentary paralysis.