If you are a loctaed in the United States, Citi Bank does not allow online gambling.
While online gambling is legal in most of the United States, a 2006 law (The UIGA) prohibits banks from allowing deposits by U.S. citizens into online gambling websites.
The way online casinos and US citizens get around this law is with the use of eWallets. An eWallet works much like PayPal, but for online gambling sites. A player will deposit money from his/her bank account or credit card, into the eWallet, and then from the eWallet into the online casino.
A common eWallet used by US citizens is MoneyLineWallet.
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If the casino says they accept Visa or MasterCard from American players, and your card does not have an international block on it (most do not) then yes you will be able to use your capital one card to deposit into an online casino.