Hall of Famer Rich Gossage wore #54 for his entire career. David Aardsma wore #54 for the Cubs in 2006.
Chris Carter wore #54 for the Red Sox in 2008.
Mike O'Connor wore #54 for the Washington Nationals in 2008.
Brian Slocum wore #54 for the Cleveland Indians in 2008.
Julian Taveras wore #54 for the Atlanta Braves in 2008.
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Hank Aaron wore it with the Atlanta Braves, he's the only person I can think of.
currently(2011) Brian Urlacher, Brian Waters. Two 54's in the Hall of Fame in The Manster Randy White and the late Jim Ringo