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During the light-dependent reactions, ATP is produced by the Photosystem II electron transport chain. According to the chemiosmosis theory, as the electrons are transported down the electron transport chain, some of the energy released is used to pump protons across the thylakoid membrane from the stroma of the chloroplast to the thylakoid interior space. this produces a proton concentration gradient or proton motive force. Since protons (hydrogen ions) are positively charged, this will lead to the accumulation of a positive charge in the interior of the membranes. As the accumulating protons in the thylakoid interior space pass back across the thylakoid membrane to the stroma through ATP synthetase complexes, this proton motive force is used to generate ATP from ADP and Pi For an excellent account see: http://student.ccbcmd.edu/biotutorials/photosyn/ldr.html

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16y ago
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14y ago

It becomes positive because ATP synthase allows H+ ions to pass through the membrane.

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13y ago

H posotive biulds up in the space as water is split

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12y ago

b. ATP synthase allows H+ ions to pass through the membrane.

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13y ago

H+ ions are released as water splits

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13y ago

h+ ions are released as water splits

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Q: Why does the inside of the thylakoid membrane become positively charged during light-dependent reactions?
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