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Q: How much Tris is required for 0.05M Tris solution?
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What Volume of 5M HCl to make tris buffer at pH 7.5?

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How do you prepare 0.1M tris HCl buffer of pH 8?

Tris(hydroxymethyl)aminomethane (Tris) has a molecular weight of 121.14 g/mol. 50 mM = 0.050 mol/L (x 121.14 g/mol) = 6.057 g/L To prepare a 1L solution first weigh out 6.057 g Tris Add roughly 70% of final volume of water (i.e. 700 mL) Use a pH-meter to measure the pH of the solution Lower the pH of the solution to 7.2 using undiluted HCl Use a measuring cylinder or volumetric flask to make the volume up to 1000 mL If you add too much HCl you need to add more Tris and then recalculate the amount of water that you need add. In this case, every 1 g of Tris requires 165 mL of water to be added.


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How much solute is in solution?

The concentration of a solution is some measurement of how much solute there is in the solution.


How much solute is in a solution?

The concentration of a solution is some measurement of how much solute there is in the solution.


How much solute is in a concentrate solution'?

The concentration of a solution is some measurement of how much solute there is in the solution.


How much CuSO4.5H2O is required to prepare 100mL of a 0.050 M aqueous stock solution?

RMM of CuSo4 . 5H2O = 160 + 5x18 = 250 g How much contain gram in 100 mL of 0.050 M CuSO4 solution is - Solution: = 0.050 x 100/1000 x 250 = 0.050x4 (100)/ 16 = 1.25 g


How do you determine the concentration of solution?

To determine the concentration of a solution, you would need to separate the solution. You then determine how much of the solution is diluted, and how much is whole.