Miami men's tennis program winning 137 straight matches from 1957-1964.
Second place is Penn State womens volleyball with 109 wins.
Florida Gators
The longest winning streak in sports history is held by the New York Yachting Club which held the Americas Cup trophy for 136 years from 1851 to 1987.
Penn State - 111 matches .gopsusports.com/sports/w-volley/recaps/091110aaa.html
Wartburg Wrestling in Division III has won 20 consecutive IIAC Titles and has not lost a IIAC dual meet since 1993.
Worldcup Soccer
Jan vennegoor of hesselink
the 2009 nets went 0-18 till finally winning one
Caltech beavers 207 games division 3 basketball
Test Match Cricket is the longest game, spanning several days for a single match.
The longest running sports show is Canada's Hockey Night in Canada which debuted in 1952 and remains on the air today (as of 08/2012)
Brandon High School wrestling (Florida) hasn't lost a match in 34 years.
cleveland indians