This depends on the final volume you intend on making. Say you want to prepare 500 mL of 1X TAE. You will need 10 mL of 50X TAE to prepare 500 mL of 1X TAE.
The main difference is in composition. In TE common Tris buffer is bring down to pH 8 with HCl and EDTA is involved but in TAE instead of Tris HCl in TE Tris-acetate buffer is used.
the function of a buffer is to maintain the pH of the sample.
function of a frame buffer in computer?
to make 500ml of 1x TAE solution we have to take 5ml of 100x TAE solution. mix it in 495 ml of deionized water.
Usually you need to dilute from a 50x stock. On that case dilute: For 500mL: 10mL 50x TAE + 490mL H2O For 1L: 20mL 50x TAE + 980mL H2O
0.04 M Tris-acetate, 0.001 M EDTA
tae mo
tae :)
It act as a buffer in Northern blotting.
We use it for isolation of proteins from yeast cells as a lysis buffer
tae