Can't say I'm 100% sure, but I think it's the Martin clippers with 37 regular season losses.
Auburn 18
fifteen
Boston
Longest College Football Losing StreaksAs of Oct. 2013, Southern Miss. has the longest losing streak at 0-18 & It extends back to 2 years ago (Starting in 2012-13 school year). New Mexico has the 2nd.
Longest winning streak is 64 games starting in the 1966 season at Pittsfield High School, Pittsfield, IL
Check out the 'Consecutive Games Played Streaks' link on this page to see the 15 longest consecutive games played streaks in MLB history.
From 1998-2001, Interlachen lost 38 games in a row which put them in the top three all-time for state losing streaks.
Hawthorne High School,CA 49 game losing steak even MTV Made a show about them Called the Streak.
portland they have like 800
Penn State
The longest streak is held by the University of North Carolina with 27 straight appearances in the NCAA tourney from 1975 (the first year that competition allowed more than one team from a conference to get a guaranteed bid) to 2001-the longest such streak in tournament history. Other streaks including the 2010 NCAA tournament, are Kansas (21)*, Duke (15), Michigan State (13), Gonzaga (12) Texas (12) Wisconsin (12), Pittsburgh (9). *Kansas missed the tournament in 1989 due to NCAA probation but have not missed the tournament in a normal season since 1983. Arizona has the second longest streak at 25 until 2010 when they failed to make the tournament.
Kansas (64 home wins as of 12.02.10)